Selected Publications

All Publications

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G-protein-coupled receptors, Hedgehog signaling and primary cilia. Seminars in Cell and Developmental Biology.  
Mukhopadhyay S and Rohatgi R.
EPub prior to print (2014). PMID 24845016.

Tracking the subcellular fate of 20(S)-hydroxycholesterol with click chemistry reveals a transport pathway to the Golgi.
Peyrot SM, Nachtergaele S, Luchetti G, Mydock-McGrane LK, Fujiwara H, Scherrer D, Jallouk A, Schlesinger PH, Ory DS, Covey DF, Rohatgi R.
Journal of Biological Chemistry 289(16):11095-11110 (2014). PMID: 24596093.

EFCAB7 and IQCE regulate Hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia.
Pusapati GV, Hughes CE, Dorn KV, Zhang D, Sugianto P, Aravind L*, and Rohatgi R*.
Developmental Cell 28(5):483-496 (2014). PMID: 24582806.

Gli protein activity is controlled by multi-site phosphorylation in vertebrate Hedgehog signaling.
Niewiadomski P*, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R*.
Cell Reports 6(1):168-81 (2014). PMID: 24373970.

Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling.
Nachtergaele S, Whalen DM, Mydock LK, Zhao Z, Malinauskas T, Krishnan K, Ingham PW, Covey DF, Siebold C*, Rohatgi R*.
Elife 2:e01340 (2013). PMID: 24171105.

Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier.  
Lin YC, Niewiadomski P, Lin B, Nakamura H, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi R, Inoue T.
Nature Chemical Biology 9(7):437-443 (2013).  PMID: 23666116.

Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips.
Earhart CM, Hughes CE, Gaster RS, Ooi CC, Wilson RJ, Zhou LY, Humke EW, Xu L, Wong DJ, Willingham SB, Schwartz EJ, Weissman IL, Jeffrey SS, Neal JW, Rohatgi R, Wakelee HA, Wang SX.
Lab on a Chip. 14(1), 78-88 (2013). PMID: 23969419.

Cancer risk after use of recombinant bone morphogenetic protein-2 for spinal arthrodesis.
Carragee EJ, Chu G, Rohatgi R, Hurwitz EL, Weiner BK, Yoon ST, Comer G, Kopjar B.
Journal of Bone and Joint Surgery 95(17), 1537-45 (2013). PMID: 24005193.

A Smoothened-Evc2 complex transduces the Hedgehog signal at primary cilia.  
Dorn K, Hughes CE and Rohatgi R.  
Developmental Cell 23(4):823-35 (2012). PMID: 22981989.

The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R.
Genes Dev. 2010; 24 (7): 670-82

Hedgehog signal transduction by smoothened: pharmacological evidence for a two-step activation process.
Rohatgi R, Milenkovic L, Corcoran RB, and Scott MP.
Proceedings of the National Academy of Sciences USA. 2009: 106 3196-3201

Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium.
Milenkovic L, Scott MP, Rohatgi R.
J Cell Biol. 2009; 187 (3): 365-74

Patched1 regulates Hedgehog signaling at the primary cilium.
Rohatgi R, Milenkovic, Scott MP.
Science. 2007; 317 (5836): 372-376

Singapore signalling: the 2012 hedgehog pathway cocktail.
Briscoe J, Rohatgi R.
EMBO Rep. 2012; 13 (7): 580-3

The ciliary membrane.
Rohatgi R, Snell WJ.
Curr Opin Cell Biol. 2010; 22 (4): 541-6

Arrestin Movement in Cilia.
Rohatgi R, Scott MP.
Science. 2008; 320 (5884): 1777-1781

Patching the gaps in Hedgehog signaling.
Rohatgi R, Scott MP.
Nat Cell Bio. 2007; 9 (9): 1005-1009

In vitro reconstitution of cdc42-mediated actin assembly using purified components.
Ho HY, Rohatgi R, Lebensohn A, Kirschner MW.
Methods in Enzymology. 2006: 406 174-190

Loss-of-function Analysis of EphA Receptors in Retinotectal mapping.
Feldheim DA, Nakamoto M, Osterfield M, Gale NW, DeChiara TM, Rohatgi R, Yancopoulos GD, Flanagan JG.
Journal of Neuroscience. 2004; 24 (10): 2542-2550

Toca-1 Mediates Cdc42- Dependent Actin Nucleation by Activating the N-WASP-WIP Complex.
Ho HY, Rohatgi R, Lebensohn A, Ma L, Li L, Gygi SP, Kirschner MW.
Cell. 2004; 118 (2): 203-216

The Mechanism of Regulation of WAVE1-induced Actin Nucleation by Rac1 and Nck.
Eden S, Rohatgi R, Podtelejnikov AV, Mann M, and Kirschner MW.
Nature. 2002; 418 (6899): 790-793

CR16 Forms a Complex with N-WASP in Brain and is a Novel Member of a Conserved Proline-Rich Actin-Binding Protein Family.
Ho HY, Rohatgi R, Ma L, and Kirschner MW.
Proceedings of the National Academy of Sciences USA. 2001; 98 (20): 11306-11311

Nck and Phosphatidylinositol 4,5 Bisphosphate Synergistically Activate Actin Polymerization Through the N-WASP-Arp2/3 Pathway.
Rohatgi R, Nollau P, Ho HY, Kirschner MW, and Mayer BJ.
Journal of Biological Chemistry. 2001; 276 (28): 26448-26452

WIP Regulates N-WASP-Mediated Actin Polymerization and Filopodium Formation.
Martinez-Quiles N, Rohatgi R, Anton IM, Medina M, Saville SP, Miki H, Yamaguchi H, Takenawa T, Hartwig JH, Geha RS, Ramesh N.
Nature Cell Biology. 2001; 3 (5): 484-491

Mechanism of N-WASP Activation by CDC42 and Phosphatidylinositol 4, 5-Bisphosphate.
Rohatgi R, Ho HY, Kirschner MW.
Journal of Cell Biology. 2000; 150 (6): 1299-1310

The Interaction Between N-WASP and the Arp2/3 Complex Links Cdc42-Dependent Signals to Actin Assembly.
Rohatgi R, Ma L, Miki H, Lopez M, Kirchhausen T, Takenawa T, Kirschner MW.
Cell. 1999; 97 (2): 221-231

The Arp2/3 Complex Mediates Actin Polymerization Induced by the Small GTP-Binding Protein Cdc42.
Ma L, Rohatgi R, Kirschner MW.
Proceedings of the National Academy of Sciences USA. 1998; 95 (26): 15362-15367

Kinetic and Mechanistic Analysis of Non-Enzymatic, Template-Directed Oligoribonucleotide Ligation.
Rohatgi R, Bartel DP, Szostak JW.
Journal of the American Chemical Society. 1996; 118 (14): 3332-3339

Non-Enzymatic, Template-Directed Ligation of Oligoribonucleotides is Highly Regioselective for the Formation of 3'-5'-Phosphodiester Bonds
Rohatgi R, Bartel DP, Szostak JW.
Journal of the American Chemical Society. 1996; 118 (14): 3340-3344